| A stable liver-specific urate oxidase gene knockout hyperuricemia mouse model finds activated hepatic de novo purine biosynthesis and urate nephropathy | |
| 論文作者 | Pang, L; Liang, NN; Li, CG; Merriman, TR; Zhang, H; Yan, F; Sun, WY; Li, R; Xue, XM; Liu, Z; Wang, C; Cheng, XY; Chen, ST; Yin, HY; Dalbeth, N; Yuan, X |
| 期刊/會(huì)議名稱 | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE |
| 論文年度 | 2024 |
| 論文類別 | |
| 摘要 | Urate oxidase (Uox)-deficient mice could be an optimal animal model to study hyperuricemia and associated disorders. We develop a liver-specific conditional knockout Uox-deficient (Uox CKO) mouse using the Cre/loxP gene targeting system. These Uox CKO mice spontaneously developed hyperuricemia with accumulated serum urate metabolites. Blocking urate degradation, the Uox CKO mice showed significant de novo purine biosynthesis (DNPB) in the liver along with amidophosphoribosyltransferase (Ppat). Pegloticase and allopurinol reversed the elevated serum urate (SU) levels in Uox CKO mice and suppressed the Ppat up-regulation. Although urate nephropathy occurred in 30-week-old Uox CKO mice, 90 % of Uox-deficient mice had a normal lifespan without pronounced urate transport abnormality. Thus, Uox CKO mice are a stable model of human hyperuricemia. Activated DNPB in the Uox CKO mice provides new insights into hyperuricemia, suggesting increased SU influences purine synthesis. |
| 期 | 3 |
| 卷 | 1870 |
| 影響因子 | 4.2 |
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